The present investigation was designed to appraise the ameliorative effect of ethanolic extract (HPEE) and aqueous extract (HPAE) of bark of H. pubescens on alloxan induced diabetic rats. The oral administration of ethanolic and aqueous extracts at dose of 250 and 500 mg/kg b.w. was given to alloxan (150 mg/kg b.w) induced diabetic rats for a period of 14 days. Glibenclamide (10 mg/kg b.w) was the reference drug used in the treatment. Blood glucose level was measured on 3rd and 14th day of the treatment using commercial kit. Blood samples were collected and centrifuged to separate serum for estimation of lipid profile and other biochemical parameters. Daily oral administration of H. pubescens bark extracts (250 and 500 mg/kg) and glibenclamide (10 mg/kg) significantly (p<0.001) reduced fasting blood glucose and normalized the lipid profile in comparison to diabetic control group which might be due to the presence of alkaloids, sterols and tannins that have been found to be beneficial in controlling diabetes and many other diseases. Additionally, both cardiac enzymes CKMB as well as LDH were significantly reduced in both H. pubescens and glibenclamide treated diabetic rats compared to the non-treated group indicating better control of blood sugar level. The effect of alcoholic extract 500 mg/kg was nearly equal to that of reference drug glibenclamide in most of the observation. Hence, it can be concluded that HPEE possess antihyperglycemic activity with antihyperlipidemic activity which may prove beneficial in cardiovascular complications associated with diabetes mellitus.
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